DMPO Antibody, Clone N1664A: HRP

Mouse Anti-DMPO Monoclonal IgG1

Product Specifications

Background

DMPO (5,5-dimethyl-1-pyrroline N-oxide) is a widely used spin trap compound for detecting free radicals and reactive oxygen species (ROS) in biological systems. Its low toxicity and favorable pharmacokinetics make it ideal for in vivo and in vitro studies of oxidative stress—a central mechanism in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. DMPO forms stable nitrone adducts with protein and DNA radicals, enabling precise identification of oxidative modifications that contribute to neuronal damage. These adducts serve as biomarkers for radical-induced injury, offering insights into disease progression and therapeutic response. However, the presence of nitric oxide and related reactive nitrogen species can interfere with DMPO quantification, necessitating careful experimental design. By enabling real-time detection of oxidative stress at the molecular level, DMPO supports the development of antioxidant strategies and enhances understanding of redox imbalance in neurodegeneration.

CAS Number

9007-83-4

Specifications

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Product Name Alternative

5,5-dimethyl-1-pyrroline N-oxide, 5,5-dimethyl-2- (8-octanoic acid) -1-pyrroline N-oxide, DMPO nitrone adduct, 55 dimethyl 1 pyrroline N oxide nitrone adduct

Synonyms

DMPO Antibody, Clone N1664A: HRP

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

5,5-dimethyl-2- (8-octanoic acid) -1-pyrrolone-N-oxide conjugated to Ovalbumin

Target

DMPO

Clonality

Monoclonal

Isotype

IgG1

Clone

N1664A

Conjugation

HRP

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA | AM

Validated Applications

WB,IHC,ELISA

Field of Research

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256. 7. Free Radic Biol Med. (2009) April 1; 46(7): 853–865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Background Reference 01

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2 (3) : 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256. 7. Free Radic Biol Med. (2009) April 1; 46 (7) : 853–865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.