Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

DMPO Antibody, Clone N1664A: APC

Mouse Anti-DMPO Monoclonal IgG1

Product Specifications

Background

DMPO (5,5-dimethyl-1-pyrroline N-oxide) is a widely used spin trap compound for detecting free radicals and reactive oxygen species (ROS) in biological systems. Its low toxicity and favorable pharmacokinetics make it ideal for in vivo and in vitro studies of oxidative stress—a central mechanism in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. DMPO forms stable nitrone adducts with protein and DNA radicals, enabling precise identification of oxidative modifications that contribute to neuronal damage. These adducts serve as biomarkers for radical-induced injury, offering insights into disease progression and therapeutic response. However, the presence of nitric oxide and related reactive nitrogen species can interfere with DMPO quantification, necessitating careful experimental design. By enabling real-time detection of oxidative stress at the molecular level, DMPO supports the development of antioxidant strategies and enhances understanding of redox imbalance in neurodegeneration.

CAS Number

9007-83-4

Specifications

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Product Name Alternative

5,5-dimethyl-1-pyrroline N-oxide, 5,5-dimethyl-2- (8-octanoic acid) -1-pyrroline N-oxide, DMPO nitrone adduct, 55 dimethyl 1 pyrroline N oxide nitrone adduct

Synonyms

DMPO Antibody, Clone N1664A: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

5,5-dimethyl-2- (8-octanoic acid) -1-pyrrolone-N-oxide conjugated to Ovalbumin

Target

DMPO

Clonality

Monoclonal

Isotype

IgG1

Clone

N1664A

Conjugation

APC

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA | AM

Validated Applications

IHC,ICC/IF

Field of Research

Cancer | Oxidative Stress | Cell Signaling | Cell Signaling | Metabolism | Mitochondrial Metabolism | Mitochondrial Markers | Cell Signaling | Metabolism | Redox Metabolism

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256. 7. Free Radic Biol Med. (2009) April 1; 46(7): 853–865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Product Datasheet

https://www.stressmarq.com/wp-content/uploads/2015/05/APC-Fluorescent-conjugate.pdf

Product MSDS

https://www.stressmarq.com/wp-content/uploads/Antibody-MSDS.pdf

Specificity

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

Background Reference 01

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2 (3) : 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256. 7. Free Radic Biol Med. (2009) April 1; 46 (7) : 853–865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.

Species

Species Independent

Isotype

IgG1

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Human Marapsin2 (PRSS38) activation kit by CRISPRa
GA117432 1 Kit

Human Marapsin2 (PRSS38) activation kit by CRISPRa

Ask
View Details
NF-kB p65/RelA Rabbit mAb
E45R13110N-50 50 µL

NF-kB p65/RelA Rabbit mAb

Ask
View Details
CDK12-IN-8
T207456-01 10 mg

CDK12-IN-8

Ask
View Details
CDK12-IN-8
T207456-02 50 mg

CDK12-IN-8

Ask
View Details
Brilliant Violet 421™ anti-mouse Podoplanin
GT03346 50 µg

Brilliant Violet 421™ anti-mouse Podoplanin

Ask
View Details
Intermedin (human)
5-01404 4x 1 mg

Intermedin (human)

Ask
View Details