Piccolo Antibody, Clone 6H9-B6: Biotin

Mouse Anti-Human Piccolo Monoclonal IgG2b Kappa

Product Specifications

Background

Piccolo (also known as Aczonin) is a large, multidomain scaffolding protein localized at the presynaptic active zone. It plays a central role in organizing the cytomatrix at active zones (CAZ) and coordinating synaptic vesicle trafficking, exocytosis, and endocytosis. Piccolo interacts with proteins involved in actin dynamics and clathrin-mediated endocytosis, including Abp1 and dynamin, thereby regulating synaptic vesicle recycling. It is often co-expressed with Bassoon, another CAZ protein, to maintain synaptic integrity and plasticity. Disruption of Piccolo expression or function has been linked to neurodevelopmental and neurodegenerative disorders, including schizophrenia and Parkinson’s disease. Its role in synaptic maintenance and plasticity makes Piccolo a promising target for understanding and treating synaptic dysfunction in neurological diseases.

CAS Number

9007-83-4

Specifications

Detects ~550kDa. No cross-reactivity to Bassoon.

Product Name Alternative

Protein piccolo, Aczonin, PCLO, Presynaptic cytomatrix protein, Multidomain presynaptic cytomatrix protein, Piccolo (presynaptic cytomatrix protein), PCLO_HUMAN, ACZ, Pico, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Piccolo mouse homolog of Pico

Synonyms

Piccolo Antibody, Clone 6H9-B6: Biotin

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

26875

Swiss Prot

Q9QYX7

Accession Number

NP_001104266.1

Cellular Locus

Cell Junction | Synapse

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Human full length recombinant protein (GenBank ID: BC001304.1)

Target

Piccolo

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

6H9-B6

Conjugation

Biotin

Type

Monoclonal

Applications

WB | ICC/IF | ELISA

Validated Applications

WB,IHC,ICC/IF |ELISA

Field of Research

Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~550kDa. No cross-reactivity to Bassoon.

Background Reference 01

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20 (7) : 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147 (1) : 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281 (9) :6038-6047.