HIF-1 alpha Antibody, Clone ESEE122

Mouse Anti-Mouse HIF-1 alpha Monoclonal IgG1

Product Specifications

Background

Hypoxia-inducible factor 1-alpha (HIF-1α) is a transcription factor that orchestrates cellular responses to low oxygen conditions. It regulates the expression of genes involved in angiogenesis, metabolism, and survival, making it a key player in adapting to hypoxic stress. In the brain, HIF-1α is activated under conditions of ischemia, inflammation, and mitochondrial dysfunction—hallmarks of many neurodegenerative diseases. Its activation promotes neuroprotective pathways, including increased glucose uptake, erythropoietin production, and vascular endothelial growth factor (VEGF) expression, which support neuronal survival and tissue repair. However, chronic or dysregulated HIF-1α signaling can contribute to pathological processes such as gliosis, blood-brain barrier disruption, and metabolic imbalance. In Alzheimer’s and Parkinson’s disease, altered HIF-1α activity has been linked to both protective and detrimental outcomes, depending on disease stage and cellular context. As a central regulator of the hypoxic response, HIF-1α represents a promising therapeutic target for modulating neuroinflammation, oxidative stress, and energy metabolism in neurodegenerative disorders.

CAS Number

9007-83-4

Specifications

Detects ~116kDa. Specific for HIF1Alpha.

Product Name Alternative

ARNT interacting protein, HIF1A, Hypoxia inducible factor 1 alpha, MOP1, PASD8

Synonyms

HIF 1 alpha Antibody, Clone ESEE122

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

15251

Swiss Prot

Q61221

Accession Number

NP_034561.2

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Bovine

Immunogen

Mouse recombinant fragment corresponding to amino acids 329-530

Target

HIF 1 alpha

Clonality

Monoclonal

Isotype

IgG1

Clone

ESEE122

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF | ELISA

Validated Applications

WB,IHC,ICC/IF,ELISA

Field of Research

Cancer | Oxidative Stress | Cell Signaling | Epigenetics and Nuclear Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4. 3. Jaakkola P., et al. (2001) Science 292: 468-72. 4. Maxwell P.H., et al. (1999) Nature 399: 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9. 7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.