Rhodopsin Antibody, Clone 4D2: FITC

Mouse Anti-Bovine Rhodopsin Monoclonal IgG1

Product Specifications

Background

Rhodopsin is a light-sensitive G protein-coupled receptor (GPCR) found in the rod photoreceptor cells of the retina. It plays a central role in the phototransduction cascade, converting light into electrical signals essential for vision in low-light conditions. Beyond its classical role in visual processing, rhodopsin has emerged as a key player in retinal neurodegeneration. Mutations in the RHO gene encoding rhodopsin are a leading cause of autosomal dominant retinitis pigmentosa (adRP), a progressive neurodegenerative disorder characterized by photoreceptor cell death and vision loss. Misfolded rhodopsin accumulates in the endoplasmic reticulum, triggering cellular stress responses, including the unfolded protein response (UPR) and apoptosis. Rhodopsin dysfunction also affects mitochondrial dynamics, oxidative stress regulation, and autophagy—pathways commonly implicated in broader neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Its involvement in protein misfolding and trafficking makes rhodopsin a valuable model for studying proteostasis and neurodegeneration. As a well-characterized GPCR with known structural and functional properties, rhodopsin serves as a powerful tool for investigating the molecular mechanisms underlying neuronal survival, degeneration, and therapeutic intervention strategies in neurodegenerative research.

CAS Number

9007-83-4

Specifications

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Product Name Alternative

OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4

Synonyms

Rhodopsin Antibody, Clone 4D2: FITC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

509933

Swiss Prot

P02699

Accession Number

NP_001014890.1

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Bovine,Mouse,Fish,Avian,Amphibians,Shark

Immunogen

Bovine Rhodopsin

Target

Rhodopsin

Clonality

Monoclonal

Isotype

IgG1

Clone

4D2

Conjugation

FITC

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Neuroscience | Neurotransmitter Receptors | Rhodopsins | Cell Signaling

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Background Reference 01

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48 (45) : 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46 (27) : 4556-4567.