HDEL Antibody, Clone 2E7: RPE

Mouse Anti-Yeast HDEL Monoclonal IgG2b

Product Specifications

Background

The HDEL sequence (His-Asp-Glu-Leu) is a C-terminal ER retention signal found in many soluble endoplasmic reticulum (ER) resident proteins. It plays a critical role in maintaining ER homeostasis by ensuring that chaperones and folding enzymes are retained or retrieved from the Golgi apparatus back to the ER. In neurodegenerative diseases, where ER stress and protein misfolding are prominent pathological features, the HDEL motif is essential for the proper localization and function of ER-resident proteins such as GRP78, GRP94, and protein disulfide isomerases. Disruption of HDEL-mediated retention can impair the unfolded protein response (UPR), exacerbate proteotoxic stress, and contribute to neuronal dysfunction. Moreover, the efficiency of the HDEL retrieval system may decline with age or under chronic stress, potentially leading to the mislocalization of protective chaperones and increased vulnerability to neurodegeneration. Understanding the dynamics of HDEL-mediated trafficking offers insights into ER stress regulation and may inform therapeutic strategies aimed at enhancing proteostasis in neurodegenerative disorders.

CAS Number

9007-83-4

Specifications

Detects ~78kDa.

Product Name Alternative

H-D-E-L (his-asp-glu-leu), endoplasmic reticulum, luminal ER protein retention, KDELR1, Endoplasmic reticulum retention signal

Synonyms

HDEL Antibody, Clone 2E7: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Cellular Locus

Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Drosophila,Yeast,Plant

Immunogen

Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Target

HDEL

Clonality

Monoclonal

Isotype

IgG2b

Clone

2E7

Conjugation

RPE

Type

Monoclonal

Applications

WB | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Cancer | Heat Shock | Cell Signaling | Protein Trafficking

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~78kDa.

Background Reference 01

1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62 (6) : 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26 (15) : 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.