Nitrotyrosine Antibody, Clone 39B6: RPE

Mouse Anti- Nitrotyrosine Monoclonal IgG2a

Product Specifications

Background

Nitrotyrosine is a post-translational modification formed by the nitration of tyrosine residues, often mediated by reactive nitrogen species such as peroxynitrite. It serves as a biomarker of nitrosative stress and is increasingly recognized for its role in neurodegenerative disease pathology. Nitrotyrosine-modified proteins are frequently detected in Alzheimer’s disease, Parkinson’s disease, and ALS, where they correlate with inflammation-induced tissue injury. Enzymes such as myeloperoxidase, cytochrome P450s, and superoxide dismutase catalyze tyrosine nitration, disrupting protein function and contributing to neuronal damage. As a marker of oxidative and nitrosative stress, nitrotyrosine provides mechanistic insight into redox imbalance and may serve as a diagnostic and therapeutic target in neurodegeneration.

CAS Number

9007-83-4

Specifications

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Product Name Alternative

Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine

Synonyms

Nitrotyrosine Antibody, Clone 39B6: RPE

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

3- (4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Target

Nitrotyrosine

Clonality

Monoclonal

Isotype

IgG2a

Clone

39B6

Conjugation

RPE

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA | FCM | AM

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Protein G Purified

Limit Of Detection

0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1400), IHC (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Background Reference 01

1. Girault I. et al. (2001) . Free Radical Biology and Medicine, 31 (11) : 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004) . Am J Physiol Lung Cell Mol Physiol. 287 (2) : L262-8. 3. Takemoto K. et al (2007) . Acta Med Okayama 61 (1) : 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26 (42) : 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330 (2) : 795-801.