Nitrotyrosine Antibody, Clone 39B6

Mouse Anti- Nitrotyrosine Monoclonal IgG2a

Product Specifications

Background

Nitrotyrosine is a post-translational modification formed by the nitration of tyrosine residues, often mediated by reactive nitrogen species such as peroxynitrite. It serves as a biomarker of nitrosative stress and is increasingly recognized for its role in neurodegenerative disease pathology. Nitrotyrosine-modified proteins are frequently detected in Alzheimer’s disease, Parkinson’s disease, and ALS, where they correlate with inflammation-induced tissue injury. Enzymes such as myeloperoxidase, cytochrome P450s, and superoxide dismutase catalyze tyrosine nitration, disrupting protein function and contributing to neuronal damage. As a marker of oxidative and nitrosative stress, nitrotyrosine provides mechanistic insight into redox imbalance and may serve as a diagnostic and therapeutic target in neurodegeneration.

CAS Number

9007-83-4

Specifications

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Product Name Alternative

Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine

Synonyms

Nitrotyrosine Antibody, Clone 39B6

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Host

Mouse

Species Reactivity

Species Independent

Immunogen

3- (4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Target

Nitrotyrosine

Clonality

Monoclonal

Isotype

IgG2a

Clone

39B6

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA | FCM | AM

Validated Applications

WB,IHC,ICC/IF,IP,ELISA,FCM,AM

Field of Research

Purification

Protein G Purified

Limit Of Detection

0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1400), IHC (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Background Reference 01

1. Girault I. et al. (2001) . Free Radical Biology and Medicine, 31 (11) : 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004) . Am J Physiol Lung Cell Mol Physiol. 287 (2) : L262-8. 3. Takemoto K. et al (2007) . Acta Med Okayama 61 (1) : 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26 (42) : 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330 (2) : 795-801.