P38 alpha MAPK Antibody, Clone 9F12: ATTO 488

Mouse Anti-Human p38 alpha MAPK Monoclonal IgG1

Product Specifications

Background

P38 MAPK (also known as SAPK2α) is a stress-activated kinase that orchestrates cellular responses to inflammatory and oxidative stimuli. It is activated by phosphorylation in response to diverse stressors, including hydrogen peroxide, cytokines, UV light, and chemotherapeutic agents. In neurons, p38 MAPK regulates apoptosis, synaptic remodeling, and inflammatory gene expression. Its activation leads to mitochondrial cytochrome c release and caspase-3 activation, contributing to neurodegeneration. Elevated p38 MAPK activity has been observed in Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. Given its central role in stress signaling and cell death, p38 MAPK is a validated target for therapeutic intervention in neurodegenerative and inflammatory CNS disorders.

CAS Number

9007-83-4

Specifications

Detects ~38kDa.

Product Name Alternative

CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A

Synonyms

p38 alpha (MAP Kinase) Antibody, Clone 9F12: ATTO 488

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1432

Swiss Prot

Q16539

Accession Number

NP_001306.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Full length recombinant protein expressed in E.coli cells

Target

p38 MAPK

Clonality

Monoclonal

Isotype

IgG1

Clone

9F12

Conjugation

ATTO 488

Type

Monoclonal

Applications

WB | IHC | IP | ELISA | AM

Validated Applications

IHC,ICC/IF

Field of Research

Cell Signaling | Post-translational Modifications | Phosphorylation | Cancer

Purification

Protein G Purified

Limit Of Detection

Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~38kDa.

Background Reference 01

1. Pearson, G. et al (2001) . Endocrine Reviews 22 (2) : 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1) : 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3) :1899-907. 7. Medicherla S. et al. (2006) . J Pharmacol Exp Ther.318 (1) : 99-107.