HSP40, YDJ1 Antibody, Clone 1G10.H8: APC

Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa

Product Specifications

Background

HSP40 proteins, also known as DnaJ homologs, form a large and diverse family of molecular co-chaperones defined by the presence of a conserved J-domain, typically located within the first 75 N-terminal amino acids. This domain is separated from the C-terminal region by a glycine/phenylalanine-rich linker and is critical for stimulating the ATPase activity of HSP70, thereby enhancing its ability to refold misfolded or aggregated proteins. Members of the HSP40 family, including the well-characterized Hdj1, are involved in a wide range of cellular processes such as protein folding, translocation, degradation, and the assembly of multi-protein complexes. In the nervous system, these functions are vital for maintaining proteostasis, especially under stress conditions that promote protein misfolding—a hallmark of neurodegenerative diseases like Alzheimer’s, Parkinson’s, and Huntington’s disease. HSP40-HSP70 complexes form the core of a larger chaperone network, interacting with co-factors such as Hip, Hop, HSP90, CHIP, and BAG-1 to direct client proteins toward refolding or degradation pathways. Differences among HSP40 subtypes, such as the presence of a zinc finger domain in HDJ2 and yeast Ydj1, further modulate their folding capabilities and functional specificity. Given their central role in regulating HSP70 activity and managing proteotoxic stress, HSP40 proteins are emerging as critical modulators of neuronal health and promising targets for therapeutic intervention in neurodegenerative disease research.

CAS Number

9007-83-4

Specifications

Detects ~40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

Product Name Alternative

DNAJB1, HSP40, HSPF1, HDJ1, DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, Human DnaJ protein 1, hDj-1, heat shock protein 40

Synonyms

HSP40, YDJ1 Antibody, Clone 1G10.H8: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

855661

Swiss Prot

P25491

Accession Number

NP_014335.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Yeast

Immunogen

Full length protein yeast HSP40 (YDJ1)

Target

HSP40

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1G10.H8

Conjugation

APC

Type

Monoclonal

Applications

WB | IP | ELISA

Validated Applications

IHC,ICC/IF

Field of Research

Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins

Purification

Protein G Purified

Limit Of Detection

0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:2000) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830. 5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.