FKBP52 Antibody, Clone Hi52C

Mouse Anti-Human FKBP52 Monoclonal IgG2a

Product Specifications

Background

FKBP52 (FK506-binding protein 4) is a co-chaperone that interacts with Hsp90 and steroid hormone receptors, influencing protein trafficking, microtubule dynamics, and neuronal signaling. In the nervous system, FKBP52 plays a protective role by regulating tau protein conformation and preventing its pathological aggregation. Reduced FKBP52 levels have been linked to increased tau pathology in Alzheimer’s disease, suggesting a neuroprotective function. FKBP52 also modulates axonal transport and synaptic stability, processes that are disrupted in several neurodegenerative conditions. As a regulator of both protein homeostasis and cytoskeletal integrity, FKBP52 represents a promising therapeutic target for halting or reversing neurodegenerative processes.

CAS Number

9007-83-4

Specifications

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Product Name Alternative

FK506 binding protein 4, FKBP4, FKBP59, HBI, HSP56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein

Synonyms

FKBP52 Antibody, Clone Hi52C

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

2288

Swiss Prot

Q02790

Accession Number

NP_002005.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Dog,Hamster

Immunogen

Recombinant full-length human FKBP52 protein

Target

FKBP52

Clonality

Monoclonal

Isotype

IgG2a

Clone

Hi52C

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP

Validated Applications

WB,IHC,ICC/IF,IP

Field of Research

Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Nuclear Import/Export

Purification

Protein G Purified

Limit Of Detection

0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Concentration

1 mg/ml

Dilution

WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Background Reference 01

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278 (19) : 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7) : 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101 (22) : 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146 (7) :3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.