FKBP52 Antibody, Clone Hi52C: Biotin

Mouse Anti-Human FKBP52 Monoclonal IgG2a

Product Specifications

Background

FKBP52 (FK506-binding protein 4) is a co-chaperone that interacts with Hsp90 and steroid hormone receptors, influencing protein trafficking, microtubule dynamics, and neuronal signaling. In the nervous system, FKBP52 plays a protective role by regulating tau protein conformation and preventing its pathological aggregation. Reduced FKBP52 levels have been linked to increased tau pathology in Alzheimer’s disease, suggesting a neuroprotective function. FKBP52 also modulates axonal transport and synaptic stability, processes that are disrupted in several neurodegenerative conditions. As a regulator of both protein homeostasis and cytoskeletal integrity, FKBP52 represents a promising therapeutic target for halting or reversing neurodegenerative processes.

CAS Number

9007-83-4

Specifications

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Product Name Alternative

FK506 binding protein 4, FKBP4, FKBP59, HBI, HSP56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein

Synonyms

FKBP52 Antibody, Clone Hi52C: Biotin

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

2288

Swiss Prot

Q02790

Accession Number

NP_002005.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Dog,Hamster

Immunogen

Recombinant full-length human FKBP52 protein

Target

FKBP52

Clonality

Monoclonal

Isotype

IgG2a

Clone

Hi52C

Conjugation

Biotin

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP

Validated Applications

WB,IHC,ICC/IF |ELISA

Field of Research

Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Nuclear Import/Export

Purification

Protein G Purified

Limit Of Detection

0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Concentration

1 mg/ml

Dilution

WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

Conjugated antibodies should be stored according to the product label

Specificity

Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Background Reference 01

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278 (19) : 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7) : 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101 (22) : 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146 (7) :3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.