CaMKII alpha Antibody, Clone 6G9: APC

Mouse Anti-Rat CaMKII alpha subunit Monoclonal IgG1

Product Specifications

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a pivotal serine/threonine kinase that plays a critical role in synaptic plasticity, memory formation, and neurodegenerative disease mechanisms. Highly enriched in forebrain neurons, CaMKII is localized to both the soma and dendrites, where it orchestrates calcium signaling in response to synaptic activity. The neuronal isoforms—comprising 52 kDa (α subunit) and 60 kDa (β subunit) —assemble into multimeric holoenzymes that integrate calcium dynamics with downstream signaling. CaMKII activation is initiated by Ca²⁺/calmodulin binding, which relieves autoinhibition and triggers autophosphorylation at threonine 286 (Thr286) . This phosphorylation event confers autonomous kinase activity, even after calcium levels subside, and enhances CaMKII’s affinity for NMDA receptors at postsynaptic densities—an essential mechanism for long-term potentiation (LTP) . The phosphorylation state of Thr286 is tightly regulated: protein phosphatase 1 (PP1) dephosphorylates it, while protein kinase A (PKA) counteracts this dephosphorylation, sustaining CaMKII activity. Dysregulation of CaMKII signaling has been implicated in multiple neurodegenerative disorders, including Alzheimer’s disease, where aberrant phosphorylation and synaptic dysfunction are hallmark features. As a master regulator of synaptic strength and plasticity, CaMKII remains a high-value target in neuroscience research and therapeutic development for cognitive and neurodegenerative disorders.

CAS Number

9007-83-4

Specifications

Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Product Name Alternative

CaMKII, CaM kinase II, CaM KII, CaMK-II, CamK-II, CAMKII, CaMKii, CamKII, Camkii, camKII, camkII, camkii, CAMKIIalpha, CamKIIalpha, CAMKIId, CDPK1, dCAMKII, dCaMKII, DCK, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA

Synonyms

CaMKII Antibody, Clone 6G9: APC

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

12322

Swiss Prot

P11798

Accession Number

NP_033922.1

Cellular Locus

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Bovine

Immunogen

Partially purified rat CaMKII

Target

CaMKII

Clonality

Monoclonal

Isotype

IgG1

Clone

6G9

Conjugation

APC

Type

Monoclonal

Applications

WB | IHC | ICC/IF | IP | ELISA | RIA

Validated Applications

IHC,ICC/IF

Field of Research

Cell Signaling | Post-translational Modifications | Phosphorylation

Purification

Protein G Purified

Limit Of Detection

0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Concentration

1 mg/ml

Dilution

WB (1:10000), IHC (1:2000), ICC/IF (1:50) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

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