Biotinylated Human IL-31 Protein, His, Avitag™ (MALS verified)

IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. May function in skin immunity. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 27-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q6EBC2-1

Expression Region

Ser 24 - Thr 164

Host

HEK293

Target

IL-31

Conjugation

Biotin-labeled

Tag

N-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human IL-31 Protein, His,Avitag (Cat. No. IL1-H82E6) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-31 RA, Fc Tag (Cat. No. ILA-H5256) with a linear range of 0.005-1.25 μg/mL (QC tested).