Monkeypox virus (Democratic Republic of the Congo) E8L Protein, His Tag

Monkeypox virus (Democratic Republic of the Congo) E8L Protein, His Tag (E8L-M52H4) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Thr 275 (Accession # EPI_ISL_19093812, GISAID) .

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. E8L can Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 38-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI_ISL_19093812

Expression Region

Met 1 - Thr 275

Host

HEK293

Target

E8L (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (Democratic Republic of the Congo)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (Democratic Republic of the Congo) E8L Protein, His Tag (Cat. No. E8L-M52H4) at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV E8L Antibody with a linear range of 0.1-3 ng/mL (QC tested).