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Human CD1d&B2M Monomer Protein (Peptide free, MALS verified)

CD1d is a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. They are non-classical MHC proteins, related to the class I MHC proteins, and are involved in the presentation of lipid antigens to T cells. CD1d is the only member of the group 2 CD1 molecules. CD1d-presented lipid antigens activate a special class of T cells, known as natural killer T (NKT) cells, through the interaction with the T-cell receptor present on NKT membranes. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and interleukin 4 production.

Product Specifications

Background

CD1d is a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. They are non-classical MHC proteins, related to the class I MHC proteins, and are involved in the presentation of lipid antigens to T cells. CD1d is the only member of the group 2 CD1 molecules. CD1d-presented lipid antigens activate a special class of T cells, known as natural killer T (NKT) cells, through the interaction with the T-cell receptor present on NKT membranes. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and interleukin 4 production.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.3 kDa and 15.7 kDa. The protein migrates as 55-60 kDa and 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P15813 (CD1d) & P61769 (B2M)

Expression Region

Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M)

Host

HEK293

Target

CD1d&B2M

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human CD1d&B2M Monomer Protein (Cat. No. CDM-H52H5) at 1 μg/mL (100 μL/well) can bind Human CD1d Antibody, Mouse IgG2B with a linear range of 0.05-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

37.3 kDa and 15.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Human

Protein ID

P15813 (CD1d) & P61769 (B2M)

Preservative

Trehalose

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