Anti-Hu SIGLEC10 PE

SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

SIGLEC10 extracellular domain fused with human IgG1 Fc fragment

Target Antigen

SIGLEC10

Clone

5G6

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Stephenson HN, Mills DC, Jones H, Milioris E, Copland A, Dorrell N, Wren BW, Crocker PR, Escors D, Bajaj-Elliott M: Pseudaminic acid on Campylobacter jejuni flagella modulates dendritic cell IL-10 expression via Siglec-10 receptor: A novel flagellin-host interaction. J Infect Dis. 2014 Nov 1;210 (9) :1487-98., URL: https://pubmed.ncbi.nlm.nih.gov/24823621/, *Nguyen DH, Ball ED, Varki A: Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol. 2006 Jun;34 (6) :728-35., URL: https://pubmed.ncbi.nlm.nih.gov/16728277/, *Läubli H, Kawanishi K, Vazhappilly CG, Matar R, Merheb M, Siddiqui SS: Tools to study and target the Siglec-sialic acid axis in cancer. FEBS J. 2021 Nov;288 (21) :6206-6225., URL: https://pubmed.ncbi.nlm.nih.gov/33251699/, *Bhamidipati K, Silberstein JL, Chaichian Y, Baker MC, Lanz TV, Zia A, Rasheed YS, Cochran JR, Robinson WH: CD52 is elevated on B cells of SLE patients and regulates B cell function. Front Immunol. 2021 Feb 4;11:626820., URL: https://pubmed.ncbi.nlm.nih.gov/33658999/, *Munday J, Kerr S, Ni J, Cornish AL, Zhang JQ, Nicoll G, Floyd H, Mattei MG, Moore P, Liu D, Crocker PR: Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor. Biochem J. 2001 Apr 15;355 (Pt 2) :489-97., URL: https://pubmed.ncbi.nlm.nih.gov/11284738/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 5G6 recognizes an extracellular epitope of human SIGLEC10, a sialic acid-binding lectin expressed on subsets of human leucocytes.