Mouse Protein CYR61 (CYR61) ELISA Kit

The secreted protein encoded by CYR61 is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Protein CYR61 (CYR61) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CYR61. No significant cross-reactivity or interference between Mouse CYR61 and analogues was observed.

This Mouse Protein CYR61 (CYR61) ELISA Kit employs a two-site sandwich ELISA to quantitate CYR61 in samples. An antibody specific for CYR61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYR61 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYR61 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYR61 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.