Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit

L1, also known as L1CAM, is a transmembrane protein; it is a neuronal cell adhesion molecule, member of the L1 protein family, of 200-220 kDa, and involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. L1CAM has also been designated CD171 . The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin superfamily of proteins. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH.

L1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; OTTHUMP00000025992; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse L1CAM. No significant cross-reactivity or interference between Mouse L1CAM and analogues was observed.

This Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit employs a two-site sandwich ELISA to quantitate L1CAM in samples. An antibody specific for L1CAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL1CAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L1CAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L1CAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

L1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; OTTHUMP00000025992; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.