Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit

Mmp9 encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis.

MMP9; GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase; MANDP2; macrophage gelatinase; matrix metalloproteinase 9; type V collagenase

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP9. No significant cross-reactivity or interference between Mouse MMP9 and analogues was observed.

This Mouse Matrix metalloproteinase 9 (MMP9) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP9 in samples. An antibody specific for MMP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MMP9; GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase; MANDP2; macrophage gelatinase; matrix metalloproteinase 9; type V collagenase

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.