What is the catalog number for Mouse Acetylcholinesterase (ACHE) ELISA Kit?

The catalog number for Mouse Acetylcholinesterase (ACHE) ELISA Kit is KTE70540.

What sizes are available for Mouse Acetylcholinesterase (ACHE) ELISA Kit?

Mouse Acetylcholinesterase (ACHE) ELISA Kit is available in the following size(s): 48 Tests, 96 Tests, 5x 96 Tests, 50x 96 Tests.

How many pack sizes does Mouse Acetylcholinesterase (ACHE) ELISA Kit come in?

Mouse Acetylcholinesterase (ACHE) ELISA Kit is available in 4 different pack sizes.

Is Mouse Acetylcholinesterase (ACHE) ELISA Kit in stock?

Mouse Acetylcholinesterase (ACHE) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

Mouse Acetylcholinesterase (ACHE) ELISA Kit

This Mouse Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate AChE.

Product Specifications

Background

Acetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.

CAS Number

7732-18-5

Product Name Alternative

AChE; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase

Gene ID

11423

Accession Number

P21836

Reactivity

Mouse

Tag

AChE

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Mouse Acetylcholinesterase (ACHE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse AChE. No significant cross-reactivity or interference between Mouse AChE and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Mouse Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate AChE in samples. An antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

AChE; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.