Mouse Probable threonyl-tRNA synthetase 2, cytoplasmic (TARSL2) ELISA Kit

TARSL2 (Threonyl-TRNA Synthetase Like 2) is a Protein Coding gene. Among its related pathways are tRNA Aminoacylation. GO annotations related to TARSL2 include nucleotide binding and ligase activity, forming aminoacyl-tRNA and related compounds. An important paralog of TARSL2 is TARS.

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Mouse Probable threonyl-tRNA synthetase 2, cytoplasmic (TARSL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TARSL2. No significant cross-reactivity or interference between Mouse TARSL2 and analogues was observed.

This Mouse Probable threonyl-tRNA synthetase 2, cytoplasmic (TARSL2) ELISA Kit employs a two-site sandwich ELISA to quantitate TARSL2 in samples. An antibody specific for TARSL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARSL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARSL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARSL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.