Mouse Serine/threonine-protein kinase TAO3 (TAOK3) ELISA Kit

The protein encoded by TAOK3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases.

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Mouse Serine/threonine-protein kinase TAO3 (TAOK3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAOK3. No significant cross-reactivity or interference between Mouse TAOK3 and analogues was observed.

This Mouse Serine/threonine-protein kinase TAO3 (TAOK3) ELISA Kit employs a two-site sandwich ELISA to quantitate TAOK3 in samples. An antibody specific for TAOK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAOK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAOK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAOK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.