Mouse Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit
This Mouse Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAOK1.
Product Specifications
Background
TAOK1 (TAO Kinase 1) is a Protein Coding gene. Diseases associated with TAOK1 include Chromosome 17Q11.2 Deletion Syndrome, 1.4-Mb. Among its related pathways are Mitotic Metaphase and Anaphase and Cell Cycle, Mitotic. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TAOK3.
CAS Number
7732-18-5
Product Name Alternative
TAOK1; FLJ14314; KIAA1361; MAP3K16; MARKK; PSK2; TAO1; STE20-like kinase; microtubule affinity regulating kinase kinase; serine/threonine kinase TAO1; serine/threonine protein kinase TAO1 homolog
Gene ID
216965
Accession Number
Q5F2E8
Reactivity
Mouse
Tag
TAOK1
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Mouse Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAOK1. No significant cross-reactivity or interference between Mouse TAOK1 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Mouse Serine/threonine-protein kinase TAO1 (TAOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAOK1 in samples. An antibody specific for TAOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
TAOK1; FLJ14314; KIAA1361; MAP3K16; MARKK; PSK2; TAO1; STE20-like kinase; microtubule affinity regulating kinase kinase; serine/threonine kinase TAO1; serine/threonine protein kinase TAO1 homolog
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/Q5F2E8
Calibration Range
Please inquire
Available Sizes
Frequently Asked Questions
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