Mouse Synaptogyrin-3 (SYNGR3) ELISA Kit

SYNGR3 encodes an integral membrane protein. The gene belongs to the synaptogyrin gene family. Like other members of the family the protein contains four transmembrane regions. The exact function of this protein is unclear. By searching an EST database for sequences related to rat and human SYNGR1, Kedra et al. (1998) identified cDNAs encoding SYNGR2 and SYNGR3. Like other members of the synaptogyrin family, the predicted 206-amino acid SYNGR3 protein contains 4 transmembrane regions. Northern blot analysis revealed that the 2.2-kb SYNGR3 mRNA is expressed in brain and placenta only.By fluorescence in situ hybridization, Kedra et al. (1998) mapped the SYNGR3 gene to chromosome 16pter.

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Mouse Synaptogyrin-3 (SYNGR3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNGR3. No significant cross-reactivity or interference between Mouse SYNGR3 and analogues was observed.

This Mouse Synaptogyrin-3 (SYNGR3) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR3 in samples. An antibody specific for SYNGR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.