Mouse Synaptogyrin-1 (SYNGR1) ELISA Kit

Synaptogyrin-1 is an integral membrane protein associated with presynaptic vesicles in neuronal cells. The exact function of this protein is unclear, but studies of a similar murine protein suggest that it functions in synaptic plasticity without being required for synaptic transmission. Three alternatively spliced variants encoding three different isoforms have been identified.The predicted 1a, 1b, and 1c proteins contain 234, 191, and 192 amino acids, respectively. Northern blot analysis revealed that the 4.5-kb SYNGR1a mRNA is expressed at high levels in brain. The other transcript forms are expressed at low levels in nonneuronal tissues. In situ hybridization to embryonic and adult mouse tissues confirmed that SYNGR1a, the most abundant transcript form, shows predominantly neuronal expression.

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Mouse Synaptogyrin-1 (SYNGR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNGR1. No significant cross-reactivity or interference between Mouse SYNGR1 and analogues was observed.

This Mouse Synaptogyrin-1 (SYNGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGR1 in samples. An antibody specific for SYNGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.