Mouse Stereocilin (STRC) ELISA Kit

Stereocilin is a protein that is associated with the hair bundle of the sensory hair cells in the inner ear. The hair bundle is composed of stiff microvilli called stereocilia and is involved with mechanoreception of sound waves. This gene is part of a tandem duplication on chromosome 15; the second copy is a pseudogene. Mutations in this gene cause autosomal recessive non-syndromic deafness. Defects in STRC are a cause of non-syndromic sensorineural deafness autosomal recessive type 16 (DFNB16) . DFNB16 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Stereocilin (STRC) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse STRC. No significant cross-reactivity or interference between Mouse STRC and analogues was observed.

This Mouse Stereocilin (STRC) ELISA Kit employs a two-site sandwich ELISA to quantitate STRC in samples. An antibody specific for STRC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTRC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STRC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STRC bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.