Mouse Stathmin (STMN1) ELISA Kit

Stathmin 1/oncoprotein 18, also known as STMN1, is a highly conserved 17 kDa protein. Its function as an important regulatory protein of microtubule dynamics has been well characterized. Eukaryotic microtubules are one of three major components of the cell’s cytoskeleton. They are highly dynamic structures that continuously alternate between assembly and disassembly. Stathmin performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell’s needs. Microtubules are cylindrical polymers of α, β-tubulin. Their assembly is in part determined by the concentration of free tubulin in the cytoplasm.At low concentrations of free tubulin, the growth rate at the microtubule ends is slowed and results in an increased rate of depolymerization (disassembly) .

STMN1; RP1-125I3.5; C1orf215; FLJ32206; LAP18; Lag; MGC138869; MGC138870; OP18; PP17; PP19; PR22; SMN; OTTHUMP00000008530; leukemia-associated phosphoprotein p18; metablastin; oncoprotein 18; phosphoprotein 19

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Stathmin (STMN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse STMN1. No significant cross-reactivity or interference between Mouse STMN1 and analogues was observed.

This Mouse Stathmin (STMN1) ELISA Kit employs a two-site sandwich ELISA to quantitate STMN1 in samples. An antibody specific for STMN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTMN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STMN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STMN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

STMN1; RP1-125I3.5; C1orf215; FLJ32206; LAP18; Lag; MGC138869; MGC138870; OP18; PP17; PP19; PR22; SMN; OTTHUMP00000008530; leukemia-associated phosphoprotein p18; metablastin; oncoprotein 18; phosphoprotein 19

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.