Mouse Shadow of prion protein (SPRN) ELISA Kit

SPRN expression in human, rat, and mouse brain. The deduced human protein contains 151 amino acids. The mammalian proteins share 81 to 95% sequence identity. Alignment of all fish and mammalian Sho proteins showed that all have an N-terminal peptide sequence with an endoplasmic reticulum targeting signal for extracellular transport, a basic RG-rich region, a hydrophobic stretch in the middle of the protein that contains the same unusual composition of small aliphatic residues (GAV) as PrP and PrP-like proteins, and a C-terminal region with a putative N-glycosylation site and a possible GPI anchor site (Premzl et al., 2003, 2004) . Database searches revealed that SPRN is expressed in mouse and rat embryo, brain, and retina, in human hippocampus, and in zebrafish embryo and retina.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Shadow of prion protein (SPRN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPRN. No significant cross-reactivity or interference between Mouse SPRN and analogues was observed.

This Mouse Shadow of prion protein (SPRN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRN in samples. An antibody specific for SPRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRN bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.