Mouse Testican-2 (SPOCK2) ELISA Kit

Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK2 encodes a member of a novel Ca (2+) -binding proteoglycan family. The mature 402-amino acid protein has 5 domains: a follistatin-like domain, a Ca (2+) -binding domain, a thyroglobulin-like domain, and a C-terminal region with 2 putative glycosaminoglycan attachment sites. The SPOCK2 protein shares 43% overall sequence identity with SPOCK1. Northern blot analysis of adult mouse tissues revealed expression of a major 6.1-kb transcript at high levels in brain and at low levels in lung and testis. Minor bands of 4.4 and 2.6 kb were also detected.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Testican-2 (SPOCK2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOCK2. No significant cross-reactivity or interference between Mouse SPOCK2 and analogues was observed.

This Mouse Testican-2 (SPOCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCK2 in samples. An antibody specific for SPOCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.