Mouse Protein spinster homolog 3 (SPNS3) ELISA Kit

By searching a human database for sequences similar to the SLC22 family of anion transporters , Jacobsson et al. (2007) identified SPNS3. The deduced 512-amino acid protein has 12 putative transmembrane domains. Quantitative real-time PCR detected high expression of rat Spns3 in coronal brain sections of the olfactory bulb and frontal cortex. Expression was moderate in other coronal sections and in thymus, kidney, and spleen. Little to no expression was detected in other tissues examined. Jacobsson et al. (2007) determined that the SPNS3 gene contains 12 exons.Jacobsson et al. (2007) stated that the SPNS3 gene maps to chromosome 17p13.2; the mouse Spns3 gene maps to chromosome 11qB4.

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Mouse Protein spinster homolog 3 (SPNS3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPNS3. No significant cross-reactivity or interference between Mouse SPNS3 and analogues was observed.

This Mouse Protein spinster homolog 3 (SPNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS3 in samples. An antibody specific for SPNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.