Mouse Tight junction protein ZO-1 (TJP1) ELISA Kit

Tight junction protein ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. Tight junction (zonula occludens) protein-1 (TJP1), also referred to as ZO-1, is a 200-kD protein located on a cytoplasmic membrane surface of vertebrate intercellular tight junctions. Willott et al. (1993) isolated a full-length cDNA sequence for human TJP1. Although the function of TJP1 is unknown, the cDNA sequence predicted a multi-domain signaling protein homologous to the product of the 'discs large-1' tumor suppressor gene of Drosophila and several other membrane-associated proteins in mammals.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Tight junction protein ZO-1 (TJP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TJP1. No significant cross-reactivity or interference between Mouse TJP1 and analogues was observed.

This Mouse Tight junction protein ZO-1 (TJP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TJP1 in samples. An antibody specific for TJP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTJP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TJP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TJP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.