Mouse Transmembrane 6 superfamily member 1 (TM6SF1) ELISA Kit

TM9SF1 shows a large N-terminal hydrophilic part and a C-terminal part with nine putative hydrophobic regions characteristic of integral transmembrane domains. Computer searches with sequence databases revealed homologies with three complete yeast proteins and with at least 19 human, 10 plant and one nematode short unidentified protein sequences translated from Expressed Sequence Tags (ESTs) . Remarkably, this hMP70 protein retains between 27 and 31% overall sequence identity with the yeast proteins.Gene expression of hMP70 appears to be ubiquitous, as the mRNA is detectable in all human tissues analysed so far, as shown by Northern blot analysis. Furthermore, a protein of about 70 kDa is detectable in different mammalian cell lines, as shown by immunoblot analysis.

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Mouse Transmembrane 6 superfamily member 1 (TM6SF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM6SF1. No significant cross-reactivity or interference between Mouse TM6SF1 and analogues was observed.

This Mouse Transmembrane 6 superfamily member 1 (TM6SF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TM6SF1 in samples. An antibody specific for TM6SF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM6SF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM6SF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM6SF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.