Mouse Delta (14) -sterol reductase (TM7SF2) ELISA Kit

TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all human tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver.

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Mouse Delta (14) -sterol reductase (TM7SF2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM7SF2. No significant cross-reactivity or interference between Mouse TM7SF2 and analogues was observed.

This Mouse Delta (14) -sterol reductase (TM7SF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TM7SF2 in samples. An antibody specific for TM7SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM7SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM7SF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM7SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.