Mouse Transmembrane protein 158 (TMEM158) ELISA Kit

Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. RIS1 is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. RIS1 is not upregulated in association to any of the above-mentioned processes, but exclusively during Ras-senescence. Furthermore, RIS1 is also upregulated by the transcriptional factor Ets2, which is a known mediator of Ras-induced senescence. Interestingly, RIS1 is located at chromosomal position 3p21.3 and, more specifically, it is included in a short segment of just 1 Mb previously defined by other investigators for its tumor-suppressor activity.

TMEM158; BBP; DKFZp586E1621; RIS1; p40BBP; BINP receptor; Ras induced senescence 1; Ras-induced senescence 1; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein

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Mouse Transmembrane protein 158 (TMEM158) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM158. No significant cross-reactivity or interference between Mouse TMEM158 and analogues was observed.

This Mouse Transmembrane protein 158 (TMEM158) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM158 in samples. An antibody specific for TMEM158 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM158 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM158 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM158 bound in the initial step. The color development is stopped and the intensity of the color is measured.

TMEM158; BBP; DKFZp586E1621; RIS1; p40BBP; BINP receptor; Ras induced senescence 1; Ras-induced senescence 1; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.