Mouse Promethin (TMEM159) ELISA Kit

By PCR of a human heart cDNA library, Yu et al. (2004) cloned TMEM159, which they called promethin. The deduced 161-amino acid protein shares 70% identity with mouse promethin. Northern blot analysis revealed high expression of a 1.8-kb transcript in heart and skeletal muscle, with weak expression in kidney, small intestine, lung, and liver. Immunofluorescence microscopy localized promethin in the cytosol of transfected human cell lines. Using microarray analysis, Yu et al. (2004) found that promethin was among the genes upregulated in a mouse model of hepatic steatosis caused by Pparg overexpression on a Ppara -/- background. Promethin expression was not upregulated in hepatic steatosis induced by fasting or choline deficiency.

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Mouse Promethin (TMEM159) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM159. No significant cross-reactivity or interference between Mouse TMEM159 and analogues was observed.

This Mouse Promethin (TMEM159) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM159 in samples. An antibody specific for TMEM159 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM159 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM159 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM159 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.