Mouse Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit

TMIGD2 Contains2 Ig-like (immunoglobulin-like) domain. Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. Ig-like domains are involved in a variety of functions, including cell-cell recognition, cell-surface receptors, muscle structure and the immune system. This entry is for immunoglobulin-like domains. Studies indicate that the interactions essential for defining the structure of these beta sandwich proteins are also important in nucleation of folding, and that proteins containing this fold may share similar folding pathways even though the proteins may have low sequence homology. The fold consists of a beta-sandwich formed of 7 strands in 2 sheets with a Greek-key topology. Some members of the fold have additional strands.

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Mouse Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMIGD1. No significant cross-reactivity or interference between Mouse TMIGD1 and analogues was observed.

This Mouse Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIGD1 in samples. An antibody specific for TMIGD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMIGD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIGD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIGD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.