Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit

Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis. The deduced 421-amino acid human TMLHE and 405-amino acid rat Tmlhe proteins have calculated molecular masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. Subcellular fractionation localized rat Tmlhe to mitochondria. Rat Tmlhe was synthesized as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely through cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat Tmlhe as an 87-kD band, suggesting that Tmlhe forms a homodimer.

TMLHE; BBOX2; FLJ10727; TMLH; XAP130; butyrobetaine (gamma) ; 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate; epsilon-trimethyllysine hydroxylase

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMLHE. No significant cross-reactivity or interference between Mouse TMLHE and analogues was observed.

This Mouse Trimethyllysine dioxygenase, mitochondrial (TMLHE) ELISA Kit employs a two-site sandwich ELISA to quantitate TMLHE in samples. An antibody specific for TMLHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMLHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMLHE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMLHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

TMLHE; BBOX2; FLJ10727; TMLH; XAP130; butyrobetaine (gamma) ; 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate; epsilon-trimethyllysine hydroxylase

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.