Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) ELISA Kit

Thymopoietin is a protein involved in the induction of CD90 in the thymus. The thymopoetin (TMPO) gene encodes three alternatively spliced mRNAs encoding proteins of 75 kDa (alpha), 51 kDa (beta) and 39 kDa (gamma) which are ubiquitously expressed in all cells. The human TMPO gene maps to chromosome band 12q22 and consists of eight exons. TMPO alpha is present diffusely expressed with the cell nucleus while TMPO beta and gamma are localized to the nuclear membrane. TMPO beta is a human homolog of the murine protein LAP2 (lamina-associated polypeptide 2) . LAP2 plays a role in the regulation of nuclear architecture by binding lamin B1 and chromosomes. This interaction is regulated by phosphorylation during mitosis.

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Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMPO. No significant cross-reactivity or interference between Mouse TMPO and analogues was observed.

This Mouse Lamina-associated polypeptide 2, isoform alpha (TMPO) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPO in samples. An antibody specific for TMPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.