Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit

Tumor necrosis factor receptor superfamily member 17 is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b, and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.The patient from whose tumor cells the 4;16 translocation was derived had a chronic intestinal malabsorption syndrome. Histologic and immunohistochemical studies demonstrated a lymphoproliferative syndrome of mature T cells.

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Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFRSF17. No significant cross-reactivity or interference between Mouse TNFRSF17 and analogues was observed.

This Mouse Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF17 in samples. An antibody specific for TNFRSF17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.