Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit

Tumor necrosis factor ligand superfamily member 12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.The deduced in-frame fusion protein, which the authors called TWEPRIL, has a calculated molecular mass of 38 kD. TWEPRIL begins at the initiation codon of TWEAK and contains the intracellular, transmembrane, and stalk regions of TWEAK fused to the extracellular receptor-binding domain of APRIL.

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Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF12. No significant cross-reactivity or interference between Mouse TNFSF12 and analogues was observed.

This Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF12 in samples. An antibody specific for TNFSF12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.