Mouse Transportin-2 (TNPO2) ELISA Kit

Transportin-2 (KPNB2) interacts directly and specifically with M9, the bidirectional transport signal of the nuclear shuttling protein hnRNPA1 and mediates hnRNPA1 nuclear import. In the course of isolating additional transportin-1 cDNAs, Siomi et al. (1997) isolated cDNAs encoding a related protein that they designated 'transportin-2.' The sequence of the predicted 894-amino acid protein shares 84% identity with that of transportin-1. One notable difference is that transportin-2 contains a short extra sequence within the region corresponding to the M9-interacting domain of transportin-1. Far Western blotting showed that transportin-2 and transportin-1 have different substrate specificities. Siomi et al. (1997) suggested that the insert in transportin-2 modifies its interaction with import substrates.

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Mouse Transportin-2 (TNPO2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNPO2. No significant cross-reactivity or interference between Mouse TNPO2 and analogues was observed.

This Mouse Transportin-2 (TNPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO2 in samples. An antibody specific for TNPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.