Mouse TOM1-like protein 2 (TOM1L2) ELISA Kit

The protein import machinery of the mitochondrial outer membrane is comprised of a dynamic complex of proteins that mediates translocation of cytosolic precursor proteins into or across the membrane. Several proteins within this complex have been identified, such as Mas20p in yeast and MOM19 in Neurospora crassa . The 145-amino acid human polypeptide shares high similarity with Mas20p and MOM19 within the N-terminal region, but exhibits only weak homology to the tetratricopeptide-repeat B domain that is found in the other 2 proteins. The authors showed that human Mas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria in the N (in) -to-C (cyto) orientation.

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Mouse TOM1-like protein 2 (TOM1L2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOM1L2. No significant cross-reactivity or interference between Mouse TOM1L2 and analogues was observed.

This Mouse TOM1-like protein 2 (TOM1L2) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1L2 in samples. An antibody specific for TOM1L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOM1L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.