Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit

TRPM4 has 1,040 amino acids and contains 6 putative transmembrane domains. Northern blot analysis detected TRPM4 expression in most adult tissues tested, with highest levels in heart, prostate, and colon. In fetus, TRPM4 expression was most abundant in kidney. There were at least 2 distinct bands detected (6.2 and 4.2 kb), indicating alternative splicing of TRPM4. In addition, a smaller but relatively abundant 2.4-kb transcript was detected in testis.The shorter protein results from alternative splicing, with the third and fourth exons being spliced out and the beginning of the first exon being truncated. As a consequence, the first in-frame methionine lies in the fifth exon, resulting in the deletion of the first 174 amino acids.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPM4. No significant cross-reactivity or interference between Mouse TRPM4 and analogues was observed.

This Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.