Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit

The amount of PPARG, PPARG coactivator-1-alpha (PPARGC1A), ubiquinol-cytochrome c oxidoreductase core 2 subunit (UQCRC2), cytochrome c oxidase subunit I (MTCO1), uncoupling protein-2 (UCP2), and ATP synthase H (+) -transporting mitochondrial F1 complex (F1-ATP synthase) were markedly reduced in the low capacity runner rats in comparison with the high capacity runners. The uniform decline in these proteins was consistent with the hypothesis that reduced aerobic metabolism plays a causal role in the development of the differences between the low capacity runner and high capacity runner rats. impairment of mitochondrial function may link reduced fitness to cardiovascular and metabolic disease. Organism species: Homo sapiens (Human)

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Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UQCRC2. No significant cross-reactivity or interference between Mouse UQCRC2 and analogues was observed.

This Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCRC2 in samples. An antibody specific for UQCRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.