Human Relaxin (RLN) ELISA Kit

Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man.The secretion of the hormone into the blood stream just before parturition results in a marked softening and lengthening of the pubic symphysis and a softening of the cervix, which facilitates the birth process. By inhibiting uterine contractions, relaxin may influence the timing of parturition. Like insulin, relaxin consists of two peptide chains, A and B, covalently linked by disulfide bonds. By further analogy to insulin, the two peptides are synthesized as a single-chain precursor polypeptide with the B chain at the NH2-terminus.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Relaxin (RLN) ELISA Kit has high sensitivity and excellent specificity for detection of Human RLN1. No significant cross-reactivity or interference between Human RLN1 and analogues was observed.

This Human Relaxin (RLN) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN1 in samples. An antibody specific for RLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RLN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.