Human Epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit

Epidermal growth factor receptor 2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.

ERBB2; CD340; HER-2; HER-2/neu; HER2; NEU; NGL; TKR1; c-erb B2/neu protein; erbB-2; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ERBB2. No significant cross-reactivity or interference between Human ERBB2 and analogues was observed.

This Human Epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit employs a two-site sandwich ELISA to quantitate ERBB2 in samples. An antibody specific for ERBB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyERBB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERBB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERBB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ERBB2; CD340; HER-2; HER-2/neu; HER2; NEU; NGL; TKR1; c-erb B2/neu protein; erbB-2; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.