Human UDP-glucose ceramide glucosyltransferase (UGCG) ELISA Kit

Glycosphingolipids (GSLs) are a group of membrane components that contain lipid and sugar moieties. They are present in essentially all animal cells and are believed to have important roles in various cellular processes. UDP-glucose ceramide glucosyltransferase (UGCG) catalyzes the first glycosylation step in GSL biosynthesis. The product, glucosylceramide, is the core structure of more than 300 GSLs. Ichikawa et al. cloned a UGCG cDNA by expressing a human melanoma cell cDNA library in a UGCG-deficient cell line and screening for UGCG complementation. The predicted 394-amino acid UGCG protein has a potential signal-anchor sequence and a single transmembrane domain near the N terminus, and very hydrophobic regions close to the C terminus, which may interact with the membrane.

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Human UDP-glucose ceramide glucosyltransferase (UGCG) ELISA Kit has high sensitivity and excellent specificity for detection of Human UGCG. No significant cross-reactivity or interference between Human UGCG and analogues was observed.

This Human UDP-glucose ceramide glucosyltransferase (UGCG) ELISA Kit employs a two-site sandwich ELISA to quantitate UGCG in samples. An antibody specific for UGCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUGCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UGCG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UGCG bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.