Human Claudin-8 (CLDN8) ELISA Kit

Claudins, such as CLDN8, are components of epithelial cell tight junctions. Tight junctions regulate movement of solutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of the apical and basolateral plasma membrane domains. Cldn8 expression reduced paracellular permeability to monovalent inorganic and organic cations and to divalent cations, but not to anions or neutral substrates. The results were consistent with a model in which CLDN2 encodes a highly cation-permeable channel, whereas CLDN8 acts primarily as a cation barrier. Cldn8 was expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Claudin-8 (CLDN8) ELISA Kit has high sensitivity and excellent specificity for detection of Human CLDN8. No significant cross-reactivity or interference between Human CLDN8 and analogues was observed.

This Human Claudin-8 (CLDN8) ELISA Kit employs a two-site sandwich ELISA to quantitate CLDN8 in samples. An antibody specific for CLDN8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCLDN8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLDN8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLDN8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.