Human Platelet-derived growth factor subunit A (PDGFA) ELISA Kit

Platelet-derived growth factor subunit A is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.

PDGFA; PDGF-A; PDGF1; PDGF A-chain; platelet-derived growth factor alpha; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Platelet-derived growth factor subunit A (PDGFA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGFA. No significant cross-reactivity or interference between Human PDGFA and analogues was observed.

This Human Platelet-derived growth factor subunit A (PDGFA) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFA in samples. An antibody specific for PDGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.

PDGFA; PDGF-A; PDGF1; PDGF A-chain; platelet-derived growth factor alpha; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.